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maXis II ETD LCMS
Bruker Daltonik

Specifications:
Type: Ultra-High Resolution UHR-Q-TOF Analyzer
Quadrupole Isolation: up to 3000 m/z
Detection range: 50-40000 m/z
Full Sensitivity Resolution: 80,000 FSR
Electro Spray Ionization (ESI + NanoESI Source incl. NanoBooster dopant delivery)
MS/MS-Mode: Fragmentation via CID+ETD (parallel in the same run)
Coupled LC: Elute UHPLC (Bruker)

Selected Applications:
Mass determination of large intact proteins (up to 200 kDa)
Protein sequencing via Top-Down Mass Spectrometry of intact proteins
Mass Spectrometry of native proteins
Quantification or structure elucidation of organic compounds and metabolites (in DDA or DIA mode)

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Fluorescence Spectrometer FP-8300
JASCO

Specifications:
Cuvette based device
Monochromators: 200 - 900 nm (Ex. and Em.)
Sensitivity: >8000:1 (RMS)
Accessories:
- Polarization filters for UV-Vis range
- Temperature-controlled single cell holder
- Magnetic stirrer
- A diverse set of quartz cuvettes

Selected Applications:
Fluorescence Intensity-based enzyme and substrate kinetics (FI)
Anisotropy-based binding studies via fluorescence polarization (FP)

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UV-Vis Spectrometer Lambda20
PerkinElmer

Specifications:
Cuvette based dual-beam device
Monochromators: 200 - 800 nm
Accessories:
- Single and multiple cell holders
- Temperature control (external)
- A diverse set of quartz cuvettes

Applications:
Time-resolved enzyme and substrate kinetics
Enzyme coupled assays
Absorption spectrum scanning

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BZ 9000 Fluorescence Microscope
Keyence

Specifications:
Resolution: 1360 x 1024 pixels
Bright Field (DIC)
Fluorescence Excitation Filters:
red (Cy5; 568 nm); green (GFP; 488 nm); blue (DAPI; 358 nm)
CO2- and heat-controlled chamber

Applications:
Imaging of slides and cell culture dishes (no Microtiter Plates) Scanning of small wells Multipoint Imaging Fluorescence Live Cell Imaging
Automated Cell Counting and Morphology Merge Measurements length / area / fluorescence (histograms)

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ChemoStar Touch ECL & FL Imager
INTAS

Specifications:
6.0 MPixel CCD Camera
UV + Vis transmission
Enhanced Chemiluminescence (ECL)
Fluorescence detection (LED Cube RED 609nm)

Further ECL/FL Imager in-house:
LAS 3000 (Fujifilm)
Image Quant LAS 4000 (GE Healthcare)

Selected Applications:
Quantitative Western Blotting (WB) detection
SDS-PAGE and Agarose Gel documentation
Fluorescence detection in PAGE-gels

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Applied Biosystems Quant Studio3
Thermo Scientific

Specifications:
96 Wells (reaction volume 10–100 μL)
Excitation/detection range 450–600 nm/500–640 nm
Multiplexing for up to 4 Targets
Three Independent Temperature Zones

Applications:
Quantification of gene expression levels (qPCR).
Differential Scanning Fluorometry (DSF) for indirect protein stability determination.

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NGC Quest 10 Plus
BioRad

Specifications:
Piston Gradient Pumps (up to 252 bar, 25.2 MPa)
Flow Rates: 0.001–10 ml/min
Single Channel UV Detection at 280 nm
Separate Sample Pump
Fraction Collector BioFrac

ÄKTA Start
Ge Healthcare

Specifications:
Low-pressure peristaltic pump system
Single Channel UV Detection at 280 nm
Fraction Collector Frac30
Accessories: Electrophoresis System MiniPrep Cell (BioRad)

Further available FPLC-Devices: ÄKTA Prime plus (GE Healthcare)

Applications:
Protein purification via different chromatography strategies
- Affinity (IMAC, MBP-Tag, Strep-Tag, GST-Tag)
- Ion Exchange (Anion, Cation)
- Size Exclusion Chromatography (SEC), Desalting

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epMotion 5075t
Eppendorf

Specifications/Accessories:
15 deck positions
1- and 8-Channel Pipetting Tools (1 µL-1000 µL)
Transfer gripper
Thermomix Unit (up to 1400 rpm, 10-95°C)
2 temperature-controlled positions (2-100°C)
Magnetic separation module (96well format)

Selected Applications:
Semi-Automated DNA-Miniprep up to 96 samples in parallel
Automated small-scale recombinant protein purification
Automated MALDI mass spectrometry sample preparation
High Throughput Aliquotation (up to 192 reaction tubes in parallel)

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Clean Work Cabinet
Self-construction

Specifications / Accessories:
- Aspiration unit
- Centrifuge Minispin (Eppendorf)
- Airlock for sample transfer
- Magnetic racks

Applications:
Analytical workflows for MS-samples
Pulldown experiments with magnetic beads or centrifugation

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ALPHA 1-2 LD Freeze Dryer
Martin Christ

Specifications:
Temperature limit: -65°C
Vacuum limit <0.01 mbar (Pump RD-8; Vacuubrand)
Sample capacity: 8 Vacuum Adapters (for NS32/29 round flasks)
Vacuum glasses 600 mL for vials (0,5 mL – 50 mL)

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ALPHA RVC Rotational Vacuum Concentrator
Martin Christ

Specifications:
Pressure regulation 1000 – 1 mbar
Rotational speed: up to 1300 rpm
Temperature regulation: 0 - 60°C
Accessory: Alpha 1-2 cooling trap + vacuum pump (Martin Christ)

Applications:
Lyophilization of proteins, peptides or chemicals
Quick vacuum concentration of liquid samples

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Multitron Standard/ Innova 44
Infors HT / New Brunswick

Specifications:
5 Shaking incubators
Capacity 6 x 5L Erlenmeyer flasks per incubator
Temperature control: 10-45°C
Shaking speed: 30-300 rpm
Time dependent programs

Application:
Multi-liter parallel cultivation of E. coli or yeast for
recombinant protein expression.

Fermenter – Parallel Bioreaktor System
DASGIP by Eppendorf

Components / Specifications:
BioBlock - 4x 2L parallel batch reaktors (Temp and Stirring control)
TC4 - Temperature control module
MP8 - 8-chanell peristaltic pump (pH-regulation, AF, Feed)
PH4 - Sensor module for pH- and DO-sensing
OD4 - Sensor module for OD-sensing
MX4/4 - Gas mixing module equipped with pressured Air and O2

Application:
Recombinant protein expression in bakteria and yeast with high cell density.
Expression of isotope labeled proteins (e.g. 2H, 15N, 13C) or incorporation of Seleno-Methionin for structural studies with solution NMR or X-ray crystallography.
Site specific incorporation of PTMs via unnatural amino acids (UAAs) through the amber supression approach.

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CF1 High-Pressure Cell Disruptor
Constant Systems

Specifications:
min. Sample Volume: 10 mL
Pressure Limit: 2700 bar (40 KPSI) (free selection)
Processivity: 100 mL/min

SFX250 Digital Sonifier
BransonSpecifications:
max. Power: 300 W (Amplitude 10-100%)
Sample Volume: 0,5 mL – 500 mL
Single Tip and 24-Tip high throughput Adapter

Applications:
Cell disruption of bacteria and yeast
Degassing of Buffer Solutions (Sonifier)