UKE - Dean’s Office for Research

UKE Microscopy Imaging Facility (umif)

Aim of the UKE Microscopy Imaging Facility (umif) is to offer the use of light microscopes at the Campus Forschung/campus of UKE in a structural way.
Umif provides access to a variety of sophisticated imaging systems and technical know-how. Two scientists are available for scientific and technical support. By request they will also introduce users in the microscope systems.

Services

Following services are offered by umif:

Extensive introduction in the microscope systems
Providing assistance for planning experiments and analysing data
Support for all imaging-related questions
Assistance for purchasing microscope systems
Dependent on the project: Acquisition, processing and analysis of complex data

Equipment

Improvision LiveCell Spinning Disk
- Laser lines: 405nm / 488 nm / 561 nm
- Software: Volocity 6
- Applications: High-speed multiple XYZ imaging, 3D reconstruction, 3D deconvolution, 3D tracking, Förster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), photoactivation, photoconversion
- Live cell imaging: Environmental chamber with temperature, humidity and CO2 control
Leica TCS SP2
- Laser lines: Multi-Ar 458 nm/ 476 nm/ 488 nm/ Multi-Ar 496 nm/ 514 nm; HeNe 543 nm/633 nm
- Software: Leica LCS
- Applications: Confocal Laser Scanning Microscopy (CLSM), multiple XYZ imaging, fluorescence recovery after photo bleaching FRAP, Förster resonance energy transfer (FRET), spectral imaging
- Live cell imaging: Add-on environmental perfusion chamber with temperature, humidity and CO2 control
Leica TCS SP5
- Laser lines: Diode 405 nm/ Multi-Ar 458 nm/ 476 nm/ 488 nm/ 514 nm; DSS: 561 nm; HeNe 633 nm
- Software: Leica LAS
- Applications: Confocal Laser Scanning Microscopy (CLSM), multiple XYZ imaging, fluorescence recovery after photo bleaching (FRAP), Förster resonance energy transfer (FRET), photoactivation, photoconversion, spectral imaging
- Live cell imaging: Environmental chamber with temperature, humidity and CO2 control
Olympus cell^tool TIRFM System
- Laser lines: Diode 488 nm/ 561 nm
- Software: cell excellence RT
- Applications: Epifluorescence, total internal reflection fluorescence microscopy (TIRFM)
- Live cell imaging: Environmental chamber with temperature, humidity and CO2 control
Zeiss ApoTome
- Fluorescence: DAPI / GFP / RFP / Far-Red
- Software: Axiovision 4.8.2
- Applications: Structured illumination, automated multi XYZ Imaging, stitching
- Live cell imaging: Environmental chamber with temperature, humidity and CO2 control

Registration

You can book time slots or training requests at the various microscope systems through the online booking calender. If you have any questions or problems please contact the staff of the facility.